Heterotopic Partial Liver Transplantation Using Inbred Cirrhotic Rat: Concept of Host Hepatectomy / 대한이식학회지

ABSTRACT

Clinical heterotopic liver transplantation(HLT) has been carried out in the several institutions since 1962. Recently, Rotterdam group reported a successful clinical series of HLT in spite of several negative viewpoints against orthotopic liver transplantation. Among the successful HLT cases till now, several occurrences of hepatocellular carcinoma in the native liver had been reported. In the current study, a thirty percent liver was transplanted heterotopically in the dimethylnitrosoamine(DMN)-induced cirrhotic rats and the feasibility of host-hepatectomy after regeneration of the graft was tested, and we are going to introduce the concept of host liver resection(HR) to the clinical auxiliary liver transplantation. Of the thirty syngeneic partial HLT with portal blood input only, 16 rats died following transplantation and three rats dies after HR. The DMN-induced cirrhotic liver specimen showed a microscopic finding of nodular cirrhosis with thick intralobular fibrous septa. Grafts examined at three posttransplant weeks appeared architecturally normal and well regenerated and the atrophied host livers were small hard showing micronodularity on the surface. Of all the animals examined, no host liver tissues were regrowing after one month following near total HR. We conclude that HLT can be performed in the clinically relevant cirrhotic rats and HR is feasible after graft regeneration and expect that this experimental model can be used for the study of cirrhosis and HLT.