Blocking of Histamine Release and IgE Binding to FcepsilonRI on Human Basophils by Antibodies Produced in Camels
Allergy, Asthma & Immunology Research
;
: 583-589, 2015.
Artículo
en Inglés
| WPRIM
| ID: wpr-89920
ABSTRACT
PURPOSE:
The production of camel heavy-chain antihuman IgE (huIgE) that has the potential to block IgE-FcepsilonRI interaction and histamine release by basophils.METHODS:
Camels were immunized with a synthetic loop peptide (SLP) designed in a multiple antigen peptide system (MAPS) forming SLP-MAPS immunogen. Camel polyclonal antibodies (PCAs) were produced, purified, characterized using Protein A & G, ELISA, and SDS-PAGE, and tested for their potency to block passive sensitization and histamine release of human basophils using flow cytometry (FCM) and ELISA, respectively.RESULTS:
FCM data indicated that camel conventional (IgG1) and heavy chain antibodies (HCAbs; IgG2, and IgG3) had blocking activities of 43.9%, 72%, and 96.6%, respectively. Moreover, both IgG2 and IgG3 achieved remarkable inhibition rates of 93.98% and 97.05% in histamine release, respectively, whereas the IgG1inhibiting activity was 60.05%.CONCLUSIONS:
Camel PCAs produced against SLP-MAPS were capable of blocking the IgE-receptor interaction and the release of histamine by basophils with superiority to HCAbs. These findings may pave the way toward the possible use of camel anti-huIgE HCAbs as blocking antibodies in the treatment of IgE-mediated allergy and asthma.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Asma
/
Proteína Estafilocócica A
/
Basófilos
/
Inmunoglobulina E
/
Inmunoglobulina G
/
Camelus
/
Ensayo de Inmunoadsorción Enzimática
/
Anafilaxis Cutánea Pasiva
/
Histamina
/
Anticuerpos Bloqueadores
Límite:
Humanos
Idioma:
Inglés
Revista:
Allergy, Asthma & Immunology Research
Año:
2015
Tipo del documento:
Artículo
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