Performance of the PROTIA(TM) Allergy-Q(R) System in the Detection of Allergen-specific IgE: A Comparison With the ImmunoCAP(R) System
Allergy, Asthma & Immunology Research
;
: 565-572, 2015.
Artículo
en Inglés
| WPRIM
| ID: wpr-89922
ABSTRACT
PURPOSE:
The PROTIA(TM) Allergy-Q(R) enzyme immunoassay (EIA) is a recently developed screening assay for specific immunoglobulin E (sIgE) for multiple allergens. The ImmunoCAP(R) fluorescent EIA (FEIA) system is the most widely used method for sIgE detection. In this study, we evaluated the performance of the Allergy-Q(R) system compared to the ImmunoCAP(R) system.METHODS:
We compared the 2 systems using sera from 260 Korean allergy patients suffering from asthma (26.5%), allergic rhinitis (42.3%), atopic dermatitis (67.7%), and food allergy (18.1%). We compared sIgE-measurement results for 7 inhalant allergens, 5 food allergens, and 4 microorganism allergens.RESULTS:
Overall, 1,799 paired assay results were analyzed. Except mugwort and alternaria, most of the allergen-sIgE results showed intra-class correlation coefficients of >0.5. Inter-assay class associations were reliable for most allergens (gamma=0.858-0.987, P<0.001). Passing-Bablok regression analysis showed multiple differences in intercept and slope. The inter-method concordance was moderate to substantial for most allergens (kappa=0.713-0.898, P<0.001).CONCLUSIONS:
The PROTIA(TM) Allergy-Q(R) EIA system exhibited good detection performance compared to the ImmunoCAP(R) FEIA system in Korean allergic patients. However, because of methodological differences between the 2 assays, careful clinical implication is required for the interpretation of Allergy-Q(R) EIA results.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Asma
/
Inmunoglobulina E
/
Inmunoglobulinas
/
Alérgenos
/
Rinitis
/
Tamizaje Masivo
/
Técnicas para Inmunoenzimas
/
Artemisia
/
Dermatitis Atópica
/
Alternaria
Tipo de estudio:
Estudio diagnóstico
/
Estudio de tamizaje
Límite:
Humanos
Idioma:
Inglés
Revista:
Allergy, Asthma & Immunology Research
Año:
2015
Tipo del documento:
Artículo
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