Toll-like receptor 9 dependent activation of MAPK and NF-kB is required for the CpG ODN-induced matrix metalloproteinase-9 expression
Experimental & Molecular Medicine
;
: 239-245, 2007.
Artículo
en Inglés
| WPRIM
| ID: wpr-90608
ABSTRACT
Unmethylated CpG oligodeoxynucleotides (CpG ODNs) activate immune cells to produce immune mediators. This study demonstrates that in murine macrophage RAW 264.7 cells, CpG ODN-mediated matrix metalloproteinase-9 (MMP-9) expression is regulated at transcriptional level and requires de novo protein synthesis. Inhibition of ERK and p38 MAPK, but not JNK, results in significant decrease of CpG ODN-induced MMP-9 expression. We found that endosomal maturation inhibitors, chloroquine and bafilomycin A, block CpG ODN-induced ERK and p38 MAPK activation and the subsequent MMP-9 expression. We also observed that CpG ODN induces NF-kappa B activation and NF-kappa B is a downstream target of p38 MAPK. Taken together, our data demonstrate that CpG ODN triggers MMP-9 expression via TLR-9 dependent ERK and p38 MAPK activation followed by NF-kappa B activation.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Oligodesoxirribonucleótidos
/
Transducción de Señal
/
Línea Celular
/
Inducción Enzimática
/
FN-kappa B
/
Proteína Quinasa 1 Activada por Mitógenos
/
Metaloproteinasa 9 de la Matriz
/
Proteínas Quinasas Activadas por Mitógenos
/
Proteína Quinasa 3 Activada por Mitógenos
/
Proteínas Quinasas p38 Activadas por Mitógenos
Límite:
Animales
Idioma:
Inglés
Revista:
Experimental & Molecular Medicine
Año:
2007
Tipo del documento:
Artículo
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