Screening and Identification of Antigenic Proteins from the Hard Tick Dermacentor silvarum (Acari: Ixodidae)
The Korean Journal of Parasitology
;
: 789-793, 2015.
Artículo
en Inglés
| WPRIM
| ID: wpr-91220
ABSTRACT
In order to explore tick proteins as potential targets for further developing vaccine against ticks, the total proteins of unfed female Dermacentor silvarum were screened with anti-D. silvarum serum produced from rabbits. The results of western blot showed that 3 antigenic proteins of about 100, 68, and 52 kDa were detected by polyclonal antibodies, which means that they probably have immunogenicity. Then, unfed female tick proteins were separated by 12% SDS-PAGE, and target proteins (100, 68, and 52 kDa) were cut and analyzed by LC-MS/MS, respectively. The comparative results of peptide sequences showed that they might be vitellogenin (Vg), heat shock protein 60 (Hsp60), and fructose-1, 6-bisphosphate aldolase (FBA), respectively. These data will lay the foundation for the further validation of antigenic proteins to prevent infestation and diseases transmitted by D. silvarum.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Ixodidae
/
Electroforesis en Gel de Poliacrilamida
/
Espectrometría de Masas en Tándem
/
Proteínas de Artrópodos
/
Peso Molecular
/
Antígenos
Tipo de estudio:
Estudio diagnóstico
/
Estudio de tamizaje
Límite:
Animales
Idioma:
Inglés
Revista:
The Korean Journal of Parasitology
Año:
2015
Tipo del documento:
Artículo
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