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Isolation, identification and characterization of a chloramphenicol-degrading bacterium / 生物工程学报
Chinese Journal of Biotechnology ; (12): 3653-3662, 2021.
Artículo en Chino | WPRIM | ID: wpr-921454
ABSTRACT
Microorganisms are the dominant players driving the degradation and transformation of chloramphenicol (CAP) in the environment. However, little bacterial strains are able to efficiently degrade and mineralize CAP, and the CAP degrading pathways mediated by oxidative reactions remain unclear. In this study, a highly efficient CAP-degrading microbial consortium, which mainly consists of Rhodococcus (relative abundance >70%), was obtained through an enrichment process using CAP-contaminated activated sludge as the inoculum. A bacterial strain CAP-2 capable of efficiently degrading CAP was isolated from the consortium and identified as Rhodococcus sp. by 16S rRNA gene analysis. Strain CAP-2 can efficiently degrade CAP under different nutrient conditions. Based on the biotransformation characteristics of the detected metabolite p-nitrobenzoic acid and the reported metabolites p-nitrobenzaldehyde and protocatechuate by strain CAP-2, a new oxidative pathway for the degradation of CAP was proposed. The side chain of CAP was oxidized and broken to generate p-nitrobenzaldehyde, which was further oxidized to p-nitrobenzoic acid. Strain CAP-2 can be used to further study the molecular mechanism of CAP catabolism, and has the potential to be used in in situ bioremediation of CAP-contaminated environment.
Asunto(s)

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Aguas del Alcantarillado / Biodegradación Ambiental / Rhodococcus / ARN Ribosómico 16S / Cloranfenicol Tipo de estudio: Estudio diagnóstico / Estudio pronóstico Idioma: Chino Revista: Chinese Journal of Biotechnology Año: 2021 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Aguas del Alcantarillado / Biodegradación Ambiental / Rhodococcus / ARN Ribosómico 16S / Cloranfenicol Tipo de estudio: Estudio diagnóstico / Estudio pronóstico Idioma: Chino Revista: Chinese Journal of Biotechnology Año: 2021 Tipo del documento: Artículo