Your browser doesn't support javascript.
loading
Metabolic engineering of Escherichia coli for production of hydroxytyrosol / 生物工程学报
Chinese Journal of Biotechnology ; (12): 4243-4253, 2021.
Artículo en Chino | WPRIM | ID: wpr-921502
ABSTRACT
Hydroxytyrosol is an important fine chemical and is widely used in food and medicine as a natural antioxidant. Production of hydroxytyrosol through synthetic biology is of important significance. Here we cloned and functionally characterized a hydroxylase encoding gene HpaBC from Escherichia coli BL21, and both subunits of this enzyme can be successfully expressed to convert the tyrosol into hydroxytyrosol. A HpaBC gene integration expression cassette under the tac promoter was constructed, and integrated into the genome of a tyrosol hyper-producing E. coli YMG5A*R using CRISPR-Cas9 technology. Meanwhile, the pathway for production of acetic acid was deleted, resulting in a recombinant strain YMGRD1H1. Shake flask fermentation showed that strain YMGRD1H1 can directly use glucose to produce hydroxytyrosol, reaching a titer of 1.81 g/L, and nearly no by-products were detected. A titer of 2.95 g/L was achieved in a fed-batch fermentation conducted in a 5 L fermenter, which is the highest titer for the de novo synthesis of hydroxytyrosol from glucose reported to date. Production of hydroxytyrosol by engineered E. coli lays a foundation for further construction of hydroxytyrosol cell factories with industrial application potential, adding another example for microbial manufacturing of aromatic compounds.
Asunto(s)

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Alcohol Feniletílico / Escherichia coli / Fermentación / Ingeniería Metabólica / Glucosa Idioma: Chino Revista: Chinese Journal of Biotechnology Año: 2021 Tipo del documento: Artículo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Alcohol Feniletílico / Escherichia coli / Fermentación / Ingeniería Metabólica / Glucosa Idioma: Chino Revista: Chinese Journal of Biotechnology Año: 2021 Tipo del documento: Artículo