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Panax Notoginseng Saponin Attenuates Gastric Mucosal Epithelial Cell Injury Induced by Dual Antiplatelet Drugs through COX and PI3K/Akt/ VEGF-GSK-3β-RhoA Network Pathway / 中国结合医学杂志
Chinese journal of integrative medicine ; (12): 819-824, 2021.
Artículo en Inglés | WPRIM | ID: wpr-922104
ABSTRACT
OBJECTIVE@#To elucidate the underlying mechanism of Panax notoginseng saponin (PNS) on gastric epithelial cell injury and barrier dysfunction induced by dual antiplatelet (DA).@*METHODS@#Human gastric mucosal epithelial cell (GES-1) was cultured and divided into 4 groups a control, a DA, a PNS+DA and a LY294002+PNS+DA group. GES-1 apoptosis was detected by flow cytometry, cell permeability were detected using Transwell, level of prostaglandins E2 (PGE2), 6-keto-prostaglandin F1α (6-keto-PGF1α) and vascular endothelial growth factor (VEGF) in supernatant were measured by enzyme linked immunosorbent assay (ELISA), expression of phosphatidylinositide 3-kinase (PI3K), phosphorylated-PI3K (p-PI3K), Akt, phosphorylated-Akt (p-Akt), cyclooxygenase-1 (COX-1), cyclooxygenase-2 (COX-2), glycogen synthase kinase-3β (GSK-3β) and Ras homolog gene family member A (RhoA) were measured by Western-blot.@*RESULTS@#DA induced apoptosis and hyper-permeability in GES-1, reduced supernatant level of PGE2, 6-keto-PGF1α and VEGF (P<0.05). Addition of PNS reduced the apoptosis of GES-1 caused by DA, restored the concentration of PGE2, 6-keto-PGF1α and VEGF (P<0.05). In addition, PNS attenuated the alteration of COX-1 and COX-2 expression induced by DA, up-regulated p-PI3K/p-Akt, down-regulated RhoA and GSK-3β. LY294002 mitigated the effects of PNS on cell apoptosis, cell permeability, VEGF concentration, and expression of RhoA and GSK-3β significantly.@*CONCLUSIONS@#PNS attenuates the suppression on COX/PG pathway from DA, alleviates DA-induced GES-1 apoptosis and barrier dysfunction through PI3K/Akt/ VEGF-GSK-3β-RhoA network pathway.
Asunto(s)

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Saponinas / Inhibidores de Agregación Plaquetaria / Fosfatidilinositol 3-Quinasas / Proteína de Unión al GTP rhoA / Factor A de Crecimiento Endotelial Vascular / Células Epiteliales / Ciclooxigenasa 1 / Proteínas Proto-Oncogénicas c-akt / Panax notoginseng / Glucógeno Sintasa Quinasa 3 beta Límite: Humanos Idioma: Inglés Revista: Chinese journal of integrative medicine Año: 2021 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Saponinas / Inhibidores de Agregación Plaquetaria / Fosfatidilinositol 3-Quinasas / Proteína de Unión al GTP rhoA / Factor A de Crecimiento Endotelial Vascular / Células Epiteliales / Ciclooxigenasa 1 / Proteínas Proto-Oncogénicas c-akt / Panax notoginseng / Glucógeno Sintasa Quinasa 3 beta Límite: Humanos Idioma: Inglés Revista: Chinese journal of integrative medicine Año: 2021 Tipo del documento: Artículo