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Diversity of the T cell receptor β chain complementarity-determining region 3 in peripheral blood of neonates with sepsis: an analysis based on immune repertoire sequencing / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics ; (12): 1154-1160, 2021.
Artículo en Inglés | WPRIM | ID: wpr-922404
ABSTRACT
OBJECTIVES@#To investigate the diversity of peripheral blood T cell receptor (TCR) β chain complementarity-determining region 3 (CDR3) based on immune repertoire sequencing in neonates with sepsis and the possible pathogenesis of neonatal sepsis.@*METHODS@#A total of 12 neonates with sepsis were enrolled as the case group, and 9 healthy full-term infants, matched for gestational age, birth weight, and age, were enrolled as the control group. Omega nucleic acid purification kit (SQ blood DNA Kit II) was used to extract DNA from peripheral blood samples, TCR β chain CDR3 was amplified by multiplex PCR, and then high-throughput sequencing was performed for the products to analyze the diversity of TCR β chain CDR3 and the difference in expression.@*RESULTS@#The length and type of TCR β chain CDR3 were similar between the case and control groups, and Gaussian distribution was observed in both groups. With D50 and Shannon-Wiener index as the evaluation indices for diversity, the case group had a significantly lower diversity of TCR β chain CDR3 than the control group (@*CONCLUSIONS@#There is a significant change in the diversity of TCR β chain CDR3 in the peripheral blood of neonates with sepsis, suggesting that it might be associated with the immune pathogenesis of neonatal sepsis.
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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Receptores de Antígenos de Linfocitos T alfa-beta / Regiones Determinantes de Complementariedad / Secuenciación de Nucleótidos de Alto Rendimiento / Reacción en Cadena de la Polimerasa Multiplex / Sepsis Neonatal Límite: Humanos Idioma: Inglés Revista: Chinese Journal of Contemporary Pediatrics Año: 2021 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Receptores de Antígenos de Linfocitos T alfa-beta / Regiones Determinantes de Complementariedad / Secuenciación de Nucleótidos de Alto Rendimiento / Reacción en Cadena de la Polimerasa Multiplex / Sepsis Neonatal Límite: Humanos Idioma: Inglés Revista: Chinese Journal of Contemporary Pediatrics Año: 2021 Tipo del documento: Artículo