Ca2+ entry through reverse Na+ /Ca2+ exchanger in NCI-H716, glucagon-like peptide-1 secreting cells
The Korean Journal of Physiology and Pharmacology
;
: 219-225, 2022.
Artículo
en Inglés
| WPRIM
| ID: wpr-927092
ABSTRACT
Glucagon like peptide-1 (GLP-1) released from enteroendocine L-cells in the intestine has incretin effects due to its ability to amplify glucose-dependent insulin secretion. Promotion of an endogenous release of GLP-1 is one of therapeutic targets for type 2 diabetes mellitus. Although the secretion of GLP-1 in response to nutrient or neural stimuli can be triggered by cytosolic Ca2+ elevation, the stimulussecretion pathway is not completely understood yet. Therefore, the aim of this study was to investigate the role of reverse Na+ /Ca2+ exchanger (rNCX) in Ca2+ entry induced by muscarinic stimulation in NCI-H716 cells, a human enteroendocrine GLP-1 secreting cell line. Intracellular Ca2+ was repetitively oscillated by the perfusion of carbamylcholine (CCh), a muscarinic agonist. The oscillation of cytosolic Ca2+ was ceased by substituting extracellular Na+ with Li + or NMG + . KB-R7943, a specific rNCX blocker, completely diminished CCh-induced cytosolic Ca2+ oscillation. Type 1 Na+ /Ca2+ exchanger (NCX 1 ) proteins were expressed in NCI-H716 cells. These results suggest that rNCX might play a crucial role in Ca2+ entry induced by cholinergic stimulation in NCIH716 cells, a GLP-1 secreting cell line.
Texto completo:
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Índice:
WPRIM (Pacífico Occidental)
Idioma:
Inglés
Revista:
The Korean Journal of Physiology and Pharmacology
Año:
2022
Tipo del documento:
Artículo
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