Application of nanopore sequencing in environmental microbiology research / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 5-13, 2022.
Artículo
en Chino
| WPRIM
| ID: wpr-927688
ABSTRACT
The development of high-throughput sequencing techniques enabled a deeper and more comprehensive understanding of environmental microbiology. Specifically, the third-generation sequencing techniques represented by nanopore sequencing have greatly promoted the development of environmental microbiology research due to its advantages such as long sequencing reads, fast sequencing speed, real-time monitoring of sequencing data, and convenient machine carrying, as well as no GC bias and no PCR amplification requirement. This review briefly summarized the technical principle and characteristics of nanopore sequencing, followed by discussing the application of nanopore sequencing techniques in the amplicon sequencing, metagenome sequencing and whole genome sequencing of environmental microorganisms. The advantages and challenges of nanopore sequencing in the application of environmental microbiology research were also analyzed.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Microbiología Ambiental
/
Metagenoma
/
Secuenciación de Nucleótidos de Alto Rendimiento
/
Nanoporos
/
Secuenciación de Nanoporos
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2022
Tipo del documento:
Artículo
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