(+)-corynoline Regulates the Proliferation,Stemness and Apoptosis of Triple Negative Breast Cancer Cells / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 244-252, 2022.
Artículo
en Chino
| WPRIM
| ID: wpr-927872
ABSTRACT
Objective To explore the performance and mechanism of(+)-corynoline in treating triple negative breast cancer MDA-MB-436 cells and thus provide an option for the development of drugs against this cancer. Methods The viability,proliferation,apoptosis and migration/invasion of MDA-MB-436 cells treated with(+)-corynoline were detected by CCK-8 assay,colony formation assay,flow cytometry and Transwell assay,respectively.Furthermore,Western blotting was employed to determine the expression of related proteins,and RNA-Seq was performed for the MDA-MB-436 cells treated with(+)-corynoline. Results (+)-corynoline inhibited the proliferation and stemness and promoted the apoptosis of MDA-MB-436 cells.Further,(+)-corynoline may activate the oxidative phosphorylation pathway to play a role in inhibiting triple negative breast cancer. Conclusion (+)-corynoline can inhibit triple negative breast cancer cells,which helps to address the poor efficacy of existing chemotherapeutics and facilitate the development of drugs against this cancer.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Alcaloides de Berberina
/
Neoplasias de la Mama
/
Movimiento Celular
/
Apoptosis
/
Línea Celular Tumoral
/
Proliferación Celular
/
Neoplasias de la Mama Triple Negativas
Límite:
Femenino
/
Humanos
Idioma:
Chino
Revista:
Acta Academiae Medicinae Sinicae
Año:
2022
Tipo del documento:
Artículo
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