Study on critical quality attributes of Qingjin Huatan Decoction based on serum pharmacochemistry / 中国中药杂志

ABSTRACT

Qingjin Huatan Decoction is a classic prescription with the effects of clearing heat, moistening lung, resolving phlegm, and relieving cough. In order to explore the critical quality attributes of Qingjin Huatan Decoction, we identified the blood components of Qingjin Huatan Decoction by ultra-performance liquid chromatography quadrupole time of flight mass spectrometry(UPLC-Q-TOF-MS) under the following conditions, chromatographic column Acquity UPLC BEH C_(18) column(2.1 mm×100 mm, 1.7 μm); mobile phase 0.1% formic acid acetonitrile(A)-0.1% formic acid in water(B); gradient elution; flow rate 0.2 mL·min~(-1); column temperature 30 ℃; injection volume 5 μL. The electrospray ionization(ESI) source was used to collect data in both positive and negative ion modes under the following conditions, capillary voltage 3 kV for the positive ion mode and 2 kV for the negative ion mode; ion source temperature 110 ℃; cone voltage 30 V; cone gas flow rate 50 L·h~(-1); nitrogen degassing temperature 350 ℃; degassing volume flow rate 800 L·h~(-1); scanning range m/z 50-2 000. In this experiment, a total of 66 related components of Qingjin Huatan Decoction were identified, including 22 prototype components and 44 metabolites. The results of this study preliminarily revealed the pharmacodynamic material basis of Qingjin Huatan Decoction in vivo, which has provided an experimental basis for the determination of quality markers of Qingjin Huatan Decoction and the development of new drugs.