The Advances and Application of ABO Blood Group Genotyping Technology --Review / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 622-626, 2022.
Article
en Zh
| WPRIM
| ID: wpr-928764
Biblioteca responsable:
WPRO
ABSTRACT
The ABO blood group system is the most important blood group system in clinical transfusion. Serological technology is a routine method for the identification of ABO blood groups, however, which have some limitations in the identification of complicated ABO samples with weakened antigens or antibodies, abnormal plasma proteins, polyagglutination, or cold agglutinin, etc. With the development of molecular biology technology, ABO blood group gene was cloned, and ABO blood group genotyping technology based on DNA was established. The genotyping technologies with different throughputs such as PCR-SSP, Droplet-AS-PCR, PCR-RFLP, PCR-SBT, SNaPshot, MALDI-TOF MS and NGS have emerged. Genotyping has overcome the limitations of serology, and has become an indispensable method to solve difficult blood type, providing strong support for the correct identification of ABO blood group, and providing guarantee for precision blood transfusion. This review summarizes the progress and application of ABO blood group genotyping methods.
Palabras clave
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Tecnología
/
Sistema del Grupo Sanguíneo ABO
/
Tipificación y Pruebas Cruzadas Sanguíneas
/
Reacción en Cadena de la Polimerasa
/
Genotipo
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Año:
2022
Tipo del documento:
Article