The enhanced genomic 6 mA metabolism contributes to the proliferation and migration of TSCC cells / 国际口腔科学杂志·英文版
International Journal of Oral Science
;
(4): 11-11, 2022.
Artículo
en Inglés
| WPRIM
| ID: wpr-929139
ABSTRACT
In contrast to the well-established genomic 5-methylcytosine (5mC), the existence of N6-methyladenine (6 mA) in eukaryotic genomes was discovered only recently. Initial studies found that it was actively regulated in cancer cells, suggesting its involvement in the process of carcinogenesis. However, the contribution of 6 mA in tongue squamous cell carcinoma (TSCC) still remains uncharacterized. In this study, a pan-cancer type analysis was first performed, which revealed enhanced 6 mA metabolism in diverse cancer types. The study was then focused on the regulation of 6 mA metabolism, as well as its effects on TSCC cells. To these aspects, genome 6 mA level was found greatly increased in TSCC tissues and cultured cells. By knocking down 6 mA methylases N6AMT1 and METTL4, the level of genomic 6 mA was decreased in TSCC cells. This led to suppressed colony formation and cell migration. By contrast, knockdown of 6 mA demethylase ALKBH1 resulted in an increased 6 mA level, enhanced colony formation, and cell migration. Further study suggested that regulation of the NF-κB pathway might contribute to the enhanced migration of TSCC cells. Therefore, in the case of TSCC, we have shown that genomic 6 mA modification is involved in the proliferation and migration of cancer cells.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Neoplasias de la Lengua
/
Carcinoma de Células Escamosas
/
Metiltransferasa de ADN de Sitio Específico (Adenina Especifica)
/
Regulación Neoplásica de la Expresión Génica
/
Movimiento Celular
/
Línea Celular Tumoral
/
Proliferación Celular
/
Histona H2a Dioxigenasa, Homólogo 1 de AlkB
Límite:
Humanos
Idioma:
Inglés
Revista:
International Journal of Oral Science
Año:
2022
Tipo del documento:
Artículo
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