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Molecular cloning and functional characterization of an isoflavone glucosyltransferase from Pueraria thomsonii / 中国天然药物
Chinese Journal of Natural Medicines (English Ed.) ; (6): 133-138, 2022.
Artículo en Inglés | WPRIM | ID: wpr-929243
ABSTRACT
Pueraria thomsonii has long been used in traditional Chinese medicine. Isoflavonoids are the principle pharmacologically active components, which are primarily observed as glycosyl-conjugates and accumulate in P. thomsonii roots. However, the molecular mechanisms underlying the glycosylation processes in (iso)flavonoid biosynthesis have not been thoroughly elucidated. In the current study, an O-glucosyltransferase (PtUGT8) was identified in the medicinal plant P. thomsonii from RNA-seq database. Biochemical assays of the recombinant PtUGT8 showed that it was able to glycosylate chalcone (isoliquiritigenin) at the 4-OH position and glycosylate isoflavones (daidzein, formononetin, and genistein) at the 7-OH or 4'-OH position, exhibiting no enzyme activity to flavonones (liquiritigenin and narigenin) in vitro. The identification of PtUGT8 may provide a useful enzyme catalyst for efficient biotransformation of isoflavones and other natural products for food or pharmacological applications.
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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Clonación Molecular / Genisteína / Pueraria / Glucosiltransferasas / Isoflavonas Idioma: Inglés Revista: Chinese Journal of Natural Medicines (English Ed.) Año: 2022 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Clonación Molecular / Genisteína / Pueraria / Glucosiltransferasas / Isoflavonas Idioma: Inglés Revista: Chinese Journal of Natural Medicines (English Ed.) Año: 2022 Tipo del documento: Artículo