Analysis of Gene Expression in Human Dermal Fibroblasts Treated with Senescence-Modulating COX Inhibitors
Genomics & Informatics
;
: 56-64, 2017.
Artículo
en Inglés
| WPRIM
| ID: wpr-93440
ABSTRACT
We have previously reported that NS-398, a cyclooxygenase-2 (COX-2)–selective inhibitor, inhibited replicative cellular senescence in human dermal fibroblasts and skin aging in hairless mice. In contrast, celecoxib, another COX-2–selective inhibitor, and aspirin, a non-selective COX inhibitor, accelerated the senescence and aging. To figure out causal factors for the senescence-modulating effect of the inhibitors, we here performed cDNA microarray experiment and subsequent Gene Set Enrichment Analysis. The data showed that several senescence-related gene sets were regulated by the inhibitor treatment. NS-398 up-regulated gene sets involved in the tumor necrosis factor β receptor pathway and the fructose and mannose metabolism, whereas it down-regulated a gene set involved in protein secretion. Celecoxib up-regulated gene sets involved in G2M checkpoint and E2F targets. Aspirin up-regulated the gene set involved in protein secretion, and down-regulated gene sets involved in RNA transcription. These results suggest that COX inhibitors modulate cellular senescence by different mechanisms and will provide useful information to understand senescence-modulating mechanisms of COX inhibitors.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Envejecimiento
/
ARN
/
Expresión Génica
/
Envejecimiento de la Piel
/
Aspirina
/
Genes vif
/
Factor de Necrosis Tumoral alfa
/
Senescencia Celular
/
Análisis de Secuencia por Matrices de Oligonucleótidos
/
Ciclooxigenasa 2
Límite:
Animales
/
Humanos
Idioma:
Inglés
Revista:
Genomics & Informatics
Año:
2017
Tipo del documento:
Artículo
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