Detection of Donor Specific Anti-HLA Antibodies Using Antibody Monitoring System / 대한이식학회지
The Journal of the Korean Society for Transplantation
;
: 63-68, 2006.
Artículo
en Coreano
| WPRIM
| ID: wpr-93711
ABSTRACT
PURPOSE:
The antibody monitoring system (AMS, GTI Inc.) is a solid phase ELISA crossmatch test for the detection of IgG antibody to the donor-specific solubilized HLA class I and class II antigens. The objective of this study was to compare the results of AMS assay with donor specific anti-HLA IgG antibodies (DS-HLA Abs), as determined by ELISA-PRA and flowcytometric crossmatch test (FCXM).METHODS:
A total of 132 sera were tested for the presence of DS-HLA Abs by ELISA-EIA, FCXM and AMS assay.RESULTS:
DS-HLA Abs were determined in 41 serum samples by an ELISA-PRA panel and FCXM. There was a significant degree of concordance (84.8%) between the results from the FCXM and AMS (P<0.001). The sensitivity, specificity, the positive predictive value and the negative predictive value of AMS assay to detect DS-HLA Abs was 90.2%, 93.4%, 86.0%, 95.5%, respectively. The AMS is a simple, objective test and it has several advantages over the cell-based crossmatch test such as elimination of non-HLA antibody reactivity, elimination of the non-donor specific antibody reactivity, no need for viable cells, and the donor's HLA antigens can be prepared in advance.CONCLUSION:
This study suggests that AMS may be useful as a supportive crossmatch test or as a monitoring test after transplantation for detecting class I and/or class II DS-HLA Abs.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Donantes de Tejidos
/
Trasplante
/
Inmunoglobulina G
/
Ensayo de Inmunoadsorción Enzimática
/
Antígenos de Histocompatibilidad Clase II
/
Sensibilidad y Especificidad
/
Antígenos HLA
/
Anticuerpos
Tipo de estudio:
Estudio diagnóstico
Límite:
Humanos
Idioma:
Coreano
Revista:
The Journal of the Korean Society for Transplantation
Año:
2006
Tipo del documento:
Artículo
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