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The effect of tumor necrosis factor (TNF)-alpha to induce matrix metalloproteinase (MMPs) from the human dental pulp, gingival, and periodontal ligament cells / 대한치과보존학회지
Journal of Korean Academy of Conservative Dentistry ; : 26-36, 2011.
Artículo en Inglés | WPRIM | ID: wpr-94348
ABSTRACT

OBJECTIVES:

In the present study, three kinds of tissues cells (pulp, gingiva, and periodontal ligament) were investigated if those cells express MMP and TIMP when they were stimulated with neuropeptides (substance P, CGRP) or proinflammatory cytokine, TNF-alpha. MATERIALS AND

METHODS:

The cells cultured from human dental pulp (PF), gingiva (GF) and periodontal ligament were (PDLF) stimulated with Mock, SP, TNF-alpha, and CGRP for 24 hrs and 48 hrs. for an RNase protection assay and Enzyme Linked Immunosorbent Assay. Cells (PF, GF and PDLF) seeded in 100 mm culture dish were stimulated with SP (10(-5), 10(-8) M) or only with medium (Mock stimulation) for 4hrs and for 24 hrs for RNase Protection Assay, and they were stimulated with CGRP (10(-5) M) and TNF-alpha (2 ng/mL) for 24 hrs and with various concentraion of TNF-alpha (2, 10, and 100 ng/mL) for Rnase Protection Assay with a human MMP-1 probe set including MMP 1, 2, 8, 7, 8, 9, 12, and TIMP 2, 3. In addition, cells (PF, GF and PDLF) were stimulated with Mock and various concentraion of TNF-alpha(2, 10, and 100 ng/mL) for 24 hrs and with TNF-alpha(10 ng/mL) for 48 hrs, and the supernatents from the cells were collected for Enzyme Linked Immunosorbent Assay (ELISA) for MMP-1 and MMP-13.

RESULTS:

The expression of MMPs in PF, GF, PDLF after stimulation with SP and CGRP were not changed compared with Mock stimulation for 4 hrs and 24 hrs. The expression of MMP-1, -12, -13 24 hrs after stimulation with TNF-alpha were upregulated, however the expression of TIMP-3 in PF, GF, PDLF after stimulation with TNF-alpha were downregulated. TNF-alpha (2 ng/mL, 10 ng/mL, 100 ng/mL) increased MMP-1 and MMP-12 expression in PF dose dependently for 24 hrs.

CONCLUSIONS:

TNF-alpha in the area of inflammation may play an important role in regulating the remodeling of dentin, cementum, and alveolar bone.
Asunto(s)

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Ligamento Periodontal / Ribonucleasas / Semillas / Neuropéptidos / Ensayo de Inmunoadsorción Enzimática / Sustancia P / Factor de Necrosis Tumoral alfa / Inhibidor Tisular de Metaloproteinasa-3 / Metaloproteinasas de la Matriz / Cemento Dental Límite: Humanos Idioma: Inglés Revista: Journal of Korean Academy of Conservative Dentistry Año: 2011 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Ligamento Periodontal / Ribonucleasas / Semillas / Neuropéptidos / Ensayo de Inmunoadsorción Enzimática / Sustancia P / Factor de Necrosis Tumoral alfa / Inhibidor Tisular de Metaloproteinasa-3 / Metaloproteinasas de la Matriz / Cemento Dental Límite: Humanos Idioma: Inglés Revista: Journal of Korean Academy of Conservative Dentistry Año: 2011 Tipo del documento: Artículo