The Reliability of Histoculture Drug Response Assay (HDRA) in Chemosensitivity Tests for Breast Cancer / Journal of the Korean Cancer Association, 대한암학회지
Cancer Research and Treatment
;
: 392-397, 2001.
Artículo
en Coreano
| WPRIM
| ID: wpr-94697
ABSTRACT
PURPOSE:
Cancers are highly individual in their response to chemotherapy, however attempts to predict tumor response to drugs using in vitro cell culture have largely failed. A new technology, the histoculture drug response assay (HDRA), appears to have solved many previous problems. The purpose of this study is to evaluate the reliability of HDRA in a chemosensitivity test for breast cancer. MATERIALS ANDMETHODS:
Tumor specimens from breast cancer patients were evaluated by HDRA using different chemotherapeutic agents. Each specimen was tested using a blind method in order to determine the reproducibility of HDRA results for the same tissue and with a triplicated assay in order to determine reproducibility by different examiners. The evaluative power of this assay and the chemosensitivity of drugs for each specimen was determined.RESULTS:
Specimens of 92.9% (65/70) were successfully cultured and evaluated for chemosensitivity. The reproducibility of HDRA for the same tissue was 75% (100% agreement) and 100% (over 70% agreement), respectively. And the reproducibility by different examiners was 78.9% (100% agreement) and 94.7% (over 70% agreement), respectively. Each specimen demonstrated a response to at least one agent.CONCLUSION:
The evaluative power and reproducibility of HDRA were high, therefore it might serve as a reliable clinical method for chemosensitivity testing. However, there is a need for clinical trial in which patients are initially randomized for treatment either by HDRA direction or by clinician's choice.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Mama
/
Neoplasias de la Mama
/
Técnicas de Cultivo de Célula
/
Quimioterapia
Tipo de estudio:
Ensayo Clínico Controlado
Límite:
Humanos
Idioma:
Coreano
Revista:
Cancer Research and Treatment
Año:
2001
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS