Expressed sequence tags (ESTs) analysis of Acanthamoeba healyi
The Korean Journal of Parasitology
;
: 151-160, 2001.
Artículo
en Inglés
| WPRIM
| ID: wpr-94817
ABSTRACT
Randomly selected 435 clones from Acanthamoeba healyi cDNA library were sequenced and a total of 387 expressed sequence tags (ESTs) had been generated. Based on the results of BLAST search, 130 clones (34.4%) were identified as the genes encoding surface proteins, enzymes for DNA, energy production or other metabolism, kinases and phosphatases, protease, proteins for signal transduction, structural and cytoskeletal proteins, cell cycle related proteins, transcription factors, transcription and translational machineries, and transporter proteins. Most of the genes (88.5%) are newly identified in the genus Acanthamoeba. Although 15 clones matched the genes of Acanthamoeba located in the public databases, twelve clones were actin gene which was the most frequently expressed gene in this study. These ESTs of Acanthamoeba would give valuable information to study the organism as a model system for biological investigations such as cytoskeleton or cell movement, signal transduction, transcriptional and translational regulations. These results would also provide clues to elucidate factors for pathogenesis in human granulomatous amoebic encephalitis or keratitis by Acanthamoeba.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Acanthamoeba
/
Transducción de Señal
/
Biblioteca de Genes
/
Proteínas Protozoarias
/
ADN Protozoario
/
Análisis de Secuencia de ADN
/
Etiquetas de Secuencia Expresada
/
Amebiasis
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
/
Humanos
Idioma:
Inglés
Revista:
The Korean Journal of Parasitology
Año:
2001
Tipo del documento:
Artículo
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