Combination treatment of bisphosphonate (pamidronate) and Quercus infectoria semi- purified fraction promotes proliferation and differentiation of osteoblast cell via expression of Osterix and Runx2 marker

ABSTRACT

Objective: To understand the effects of combination treatment of pamidronate with isolated Quercus infectoria semi-purified fraction (QIsm-F) on human foetal osteoblast cell model (hFOB 1.19 cell line) through assessment of Runt related transcription fraction-2 (Runx2) and Osterix (Osx). Methods: The isolation and purification of QIsm-F were conducted by chromatographic technique. In order to assess relative efficacy of QIsm-F to the osteoblast model, the determination of half maximal effective concentration (EC