Evaluation of zoonotic potency of Escherichia coli O157: H7 through arbitrarily primed PCR methods
Asian Pacific Journal of Tropical Biomedicine
;
(12): 915-920, 2015.
Artículo
en Chino
| WPRIM
| ID: wpr-950985
ABSTRACT
Objective:
To evaluate the zoonotic potency of Escherichia coli O157H7 through arbitrarily primed-PCR (AP-PCR) methods as one of the DNA fingerprinting methods.Methods:
A total of 14 isolates consisted of 11 isolates originated from human feces with renal failure symptoms, 2 isolates originated from cattle feces, and 1 control isolate were used in this study. DNA of each isolate was extracted, and their profiles were studied by using AP-PCR method with M13 F and M13 R arbitrary primers.Results:
The results founded that all of 14 isolates had similarity range from 54.6% to 88.5%. Isolates KL-106(3) and KL-55(6) originated from humans showed the degree of similarity with isolates SM-25(1) and SM-7(1) originated from cattle as high as 85% and 77%, respectively.Conclusions:
The high degree of similarity between isolates originated from cattle and human indicated the high potency of zoonoses. The results also concluded AP-PCR method as a briefly fingerprinting method in order to trace the epidemiological of E. coli O157H7.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Asian Pacific Journal of Tropical Biomedicine
Año:
2015
Tipo del documento:
Artículo
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