Characterization of the salivary microbiome in healthy Thai children

ABSTRACT

Objective: To investigate the composition of the salivary microbiome of 50 healthy Thai children. Methods: A total 76 provinces in Thailand are grouped into 5 geographical clusters based on unique economics, foods and lifestyles. Geographical locations and the results of an oral assessment were also considered. Genomic DNA was extracted from stimulated sdiva samples. Subsequently, amplicon libraries were prepared by 16S Metagenomic Sequencing Library Preparation. The amplicons were sequenced using an Illumina Miseq platform followed by bioinformatics and statistical analyses. Results: The correlation between oral hygiene status and caries history varied from r