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Development and application of quantitative detection method for nervous necrosis virus (NNV) isolated from sevenband grouper Hyporthodus septemfasciatus
Asian Pacific Journal of Tropical Medicine ; (12): 742-748, 2016.
Artículo en Chino | WPRIM | ID: wpr-951351
ABSTRACT
Objective To develop the rapid and efficient quantitative detection tool for nervous necrosis virus isolated from sevenband grouper Hyporhodus septemfasciatus. Methods The viral genes of the NNV (SGYeosu08) isolated from sevenband grouper were phylogenetically analyzed. In addition, novel quantitative PCR primers based on the genomic sequence of SGYeosu08 isolate were designed and compared it with the conventional bio-assay method (TCID

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Idioma: Chino Revista: Asian Pacific Journal of Tropical Medicine Año: 2016 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Idioma: Chino Revista: Asian Pacific Journal of Tropical Medicine Año: 2016 Tipo del documento: Artículo