Phytopharmacological approach of free radical scavenging and anti-oxidative potential of eugenol and Ocimum gratissimum Linn

ABSTRACT

Objective: To evaluate phytopharmacologically eugenol and two extract products of Ocimum gratissimum Linn. (O. gratissimum) (Labiaceae) on free radical scavenging and antioxidant activity. Methods: Aqueous and methanol extract of fresh aerial part of O. gratissimum were prepared and eugenol (1-allyl-4-hydroxy-3-methoxybenzene) was isolated from fresh leaves and characterized by high performance liquid chromatography, fourier transform infrared spectroscopy, 1 h nuclear magnetic resonance. To establish the antioxidant potentiality of aqueous extract, methanol extract and eugenol, 1, 1-diphenyl-2-picrylhydrazyl radical, hydroxyl radical, nitric oxide scavenging activity, antioxidant activity by ferric thiocyanate and reducing power were measured in chemical system in vitro. Results: Significant (P<0.05) concentration-dependent free radical scavenging activity, antioxidant activity, and reducing power was observed by O. gratissimum products. Moreover, eugenol is more potent than the two extract products of O. gratissimum, but lower than potent antioxidant ascorbic acid. Conclusions: Hence, O. gratissimum presents a potentially valuable source of natural antioxidant and bioactive material.