CNS cholinergic innervation to the hippocampus in the rat using pseudorabies virus as a neurotracer
Journal of Korean Medical Science
;
: 209-213, 2001.
Artículo
en Inglés
| WPRIM
| ID: wpr-95270
ABSTRACT
The hippocampus is a central area of the memory-related neural system. Combined immunohistochemistry against choline acetyl transferase and retrograde transneuronal labelling of the pseudorabies virus were used to identify cholinergic neurons in the central nervous system projecting to the hippocampal formation of the rat. Five to ten microL of Bartha strain of pseudorabies virus were injected into the dentate gyrus, CA1 and CA3 of the hippocampus of 20 Sprague Dawley rats using stereotaxic instrument. Forty eight to 96 hr after the injection, the brains were removed and the tissue sections were processed for double immunofluorescence procedure using polyclonal antibodies against pseudorabies virus or choline acetyl transferase. The double labelled neurons were distributed at several different nuclei and the labelling patterns of three different areas of the hippocampus were similar. These data suggests that the cholinergic innervation to the hippocampus were distributed in a transsynaptic manner throughout the whole brain area.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Inmunohistoquímica
/
Colina O-Acetiltransferasa
/
Fibras Colinérgicas
/
Ratas Sprague-Dawley
/
Herpesvirus Suido 1
/
Hipocampo
/
Animales
/
Microinyecciones
/
Anticuerpos
/
Vías Nerviosas
Límite:
Animales
Idioma:
Inglés
Revista:
Journal of Korean Medical Science
Año:
2001
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS