Neuron-Like Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells
Yonsei Medical Journal
;
: 401-412, 2011.
Artículo
en Inglés
| WPRIM
| ID: wpr-95680
ABSTRACT
PURPOSE:
Mesenchymal stem cells (MSCs) are multipotent and give rise to distinctly differentiated cells from all three germ layers. Neuronal differentiation of MSC has great potential for cellular therapy. We examined whether the cluster of mechanically made, not neurosphere, could be differentiated into neuron-like cells by growth factors, such as epidermal growth factor (EGF), hepatocyte growth factor (HGF), and vascular endothelial growth factor (VEGF). MATERIALS ANDMETHODS:
BMSCs grown confluent were mechanically separated with cell scrapers and masses of separated cells were cultured to form cluster BMSCs. As described here cluster of BMSCs were differentiated into neuron-like cells by EGF, HGF, and VEGF. Differentiated cells were analyzed by means of phase-contrast inverted microscopy, reverse transcriptase-polymerase chain reaction (RT-PCR), immunofluorescence, and immunocytochemistry to identify the expression of neural specific markers.RESULTS:
For the group with growth factors, the shapes of neuron-like cells was observable a week later, and two weeks later, most cells were similar in shape to neuron-like cells. Particularly, in the group with chemical addition, various shapes of filament structures were seen among the cells. These culture conditions induced MSCs to exhibit a neural cell phenotype, expressing several neuro-glial specific markers.CONCLUSION:
bone marrow-derived mesenchymal stem cells (BMSCs) could be easily induced to form clusters using mechanical scraping, not neurospheres, which in turn could differentiate further into neuron-like cells and might open an attractive possibility for clinical cell therapy for neurodegenerative diseases. In the future, we consider that neuron-like cells differentiated from clusters of BMSCs are needed to be compared and analyzed on a physiological and molecular biological level with preexisting neuronal cells, and studies on the possibility of their transplantation and differentiation capability in animal models are further required.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Células de la Médula Ósea
/
Inmunohistoquímica
/
Diferenciación Celular
/
Células Cultivadas
/
Western Blotting
/
Factor de Crecimiento de Hepatocito
/
Técnicas de Cultivo de Célula
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
/
Factor A de Crecimiento Endotelial Vascular
/
Factor de Crecimiento Epidérmico
Tipo de estudio:
Estudio pronóstico
Límite:
Adulto
/
Humanos
Idioma:
Inglés
Revista:
Yonsei Medical Journal
Año:
2011
Tipo del documento:
Artículo
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