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Balsalazide Potentiates Parthenolide-Mediated Inhibition of Nuclear Factor-kappaB Signaling in HCT116 Human Colorectal Cancer Cells
Intestinal Research ; : 233-241, 2015.
Artículo en Inglés | WPRIM | ID: wpr-96060
ABSTRACT
BACKGROUND/

AIMS:

Balsalazide is an anti-inflammatory drug used in the treatment of inflammatory bowel disease. Balsalazide can reduce inflammatory responses via several mechanisms, including inhibition of nuclear factor-kappaB (NF-kappaB) activity. Parthenolide (PT) inhibits NF-kappaB and exerts promising anticancer effects by promoting apoptosis. The present investigated the antitumor effects of balsalazide, combined with PT, on NF-kappaB in a representative human colorectal carcinoma cell line, HCT116.

METHODS:

We counted cells and conducted annexin-V assays and cell cycle analysis to measure apoptotic cell death. Western blotting was used investigate the levels of proteins involved in apoptosis.

RESULTS:

PT and balsalazide produced synergistic anti-proliferative effects and induced apoptotic cell death. The combination of balsalazide and PT markedly suppressed nuclear translocation of the NF-kappaB p65 subunit and the phosphorylation of inhibitor of NF-kappaB. Moreover, PT and balsalazide dramatically enhanced NF-kappaB p65 phosphorylation. Apoptosis, through the mitochondrial pathway, was confirmed by detecting effects on Bcl-2 family members, cytochrome c release, and activation of caspase-3 and -8.

CONCLUSIONS:

Combination treatment with PT and balsalazide may offer an effective strategy for the induction of apoptosis in HCT116 cells.
Asunto(s)

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Fosforilación / Neoplasias Colorrectales / Enfermedades Inflamatorias del Intestino / Ciclo Celular / Línea Celular / Western Blotting / FN-kappa B / Muerte Celular / Apoptosis / Células HCT116 Límite: Humanos Idioma: Inglés Revista: Intestinal Research Año: 2015 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Fosforilación / Neoplasias Colorrectales / Enfermedades Inflamatorias del Intestino / Ciclo Celular / Línea Celular / Western Blotting / FN-kappa B / Muerte Celular / Apoptosis / Células HCT116 Límite: Humanos Idioma: Inglés Revista: Intestinal Research Año: 2015 Tipo del documento: Artículo