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ras Gene Mutations in Malignant Fibrous Histiocytoma
Korean Journal of Pathology ; : 232-237, 2001.
Artículo en Coreano | WPRIM | ID: wpr-96590
ABSTRACT

BACKGROUND:

ras gene mutations have been described in various human malignancies, suggesting that their activation may play a role in oncogenesis. However, there are few reports concerning ras gene alterations in malignant fibrous histiocytomas. We therefore designed a study to determine the prevalence and type of mutations in the first exons of H-ras and K-ras genes in these tumors.

METHODS:

Twenty-seven malignant fibrous histiocytomas were investigated by direct sequencing analysis with the automated DNA sequencing of polymerase chain reaction-amplified ras sequences.

RESULTS:

Twenty-four mutations were found in 18 (67%) of the tumors GGC to GAC transition mutations at codon 13 of K-ras (coding for aspartic acid instead of glycine) in 18 of the samples and GGC to GTC transversions at codon 12 of H-ras (coding for valine instead of glycine) in six of the lesions.

CONCLUSIONS:

Our data suggest an involvement of the ras gene mutation in conjunction with other yet unknown events in the tumorigenesis and/or progression of malignant fibrous histiocytomas. The K-ras gene activation predominated in these tumors by a mutation at codon 13. It is noteworthy that H-ras mutations were detected only in association with the lesions containing K-ras mutated genes, the significance of which remains to be determined.
Asunto(s)

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Valina / Codón / Exones / Prevalencia / Genes ras / Análisis de Secuencia de ADN / Ácido Aspártico / Histiocitoma Fibroso Maligno / Carcinogénesis Tipo de estudio: Estudio de prevalencia Límite: Humanos Idioma: Coreano Revista: Korean Journal of Pathology Año: 2001 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Valina / Codón / Exones / Prevalencia / Genes ras / Análisis de Secuencia de ADN / Ácido Aspártico / Histiocitoma Fibroso Maligno / Carcinogénesis Tipo de estudio: Estudio de prevalencia Límite: Humanos Idioma: Coreano Revista: Korean Journal of Pathology Año: 2001 Tipo del documento: Artículo