Inhibition of expression of P-selectin by antioxidant in cholesterol-fed rats
Journal of Korean Medical Science
;
: 8-14, 1999.
Artículo
en Inglés
| WPRIM
| ID: wpr-96720
ABSTRACT
Butylated hydroxytoluene (BHT) can inhibit experimental atherosclerosis in animals. Although the agent is an antioxidant, the exact mechanism of the reaction in atherosclerosis is still unknown. To investigate the effects of BHT on expression of P-selectin (PADGEM, GMP-140), intercellular adhesion molecule-1 (ICAM-1) and class II MHC (Ia) antigen, we proposed an experiment on rats. Male rats (n=18 per group) were fed either a normal cholesterol control diet, a normal cholesterol diet containing 0.5% BHT (BD), a high cholesterol diet containing 1.5% cholesterol and 0.1% sodium cholate (CD), or the CD diet containing 0.5% BHT (BCD). Rats were sacrificed after 3 days, and after 1, 2, 4, 10, and 17 weeks of dietary treatment. Although there was no gross or light microscopic atherosclerotic lesions, scanning electron microscopy revealed monocytic adhesion to aortic endothelium and mild endothelial injuries in CD and BCD groups. Immunohistochemically, the addition of BHT to a high cholesterol diet inhibited P-selectin expression but not in ICAM-1 and Ia antigen. These findings suggest that in rats, high cholesterol diets induce expression of ICAM-1, P-selectin and Ia antigen. In addition, the antiatherogenic effect of BHT may play a role in the inhibition of P-selectin.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Aorta Abdominal
/
Aorta Torácica
/
Hidroxitolueno Butilado
/
Colesterol en la Dieta
/
Microscopía Electrónica de Rastreo
/
Colesterol
/
Ratas Sprague-Dawley
/
Selectina-P
/
Animales
/
Antioxidantes
Límite:
Animales
Idioma:
Inglés
Revista:
Journal of Korean Medical Science
Año:
1999
Tipo del documento:
Artículo
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