Development and characterization of stable cell lines constitutively expressing the porcine reproductive and respiratory syndrome virus nucleocapsid protein
Journal of Veterinary Science
;
: 169-171, 2010.
Artículo
en Inglés
| WPRIM
| ID: wpr-96821
ABSTRACT
Despite global efforts to control porcine reproductive and respiratory syndrome virus (PRRSV) infection, the virus continues to cause economic problems in the swine industry worldwide. In this study, we attempted to generate and characterize a panel of stable BHK cell lines that constitutively express the nucleocapsid (N) protein of type 1 or type 2 PRRSV. The established BHK cell lines were found to react well with N-specific antibodies as well as the hyperimmune serum of pigs raised against each genotype of PRRSV. Taken together, the data implicate a potential usefulness for the newly generated stable cell lines as a diagnostic reagent for PRRSV serology.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Porcinos
/
Transfección
/
Línea Celular
/
Western Blotting
/
Virus del Síndrome Respiratorio y Reproductivo Porcino
/
Síndrome Respiratorio y de la Reproducción Porcina
/
Proteínas de la Nucleocápside
/
Genotipo
/
Anticuerpos Antivirales
Límite:
Animales
Idioma:
Inglés
Revista:
Journal of Veterinary Science
Año:
2010
Tipo del documento:
Artículo
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