Hepatocyte nuclear factor 1-alpha mutation in normal glucose-tolerant subjects and early-onset type 2 diabetic patients
The Korean Journal of Internal Medicine
; : 165-169, 2008.
Article
en En
| WPRIM
| ID: wpr-97390
Biblioteca responsable:
WPRO
ABSTRACT
BACKGROUND/AIMS: The prevalence of diabetes in Korea is reported to be approximately 10%, but cases of maturity-onset diabetes of the young (MODY) are rare in Korea. A diagnostic technique for autosomal dominant MODY is being actively sought. In this regard, we used a DNA chip to investigate the frequency of mutations of the MODY3 gene (hepatocyte nuclear factor-1alpha) in Korean patients with early-onset type 2 diabetes. METHODS: The genomic DNA of 30 normal individuals [age, 24.9+/-8.6 years] and 25 patients with early-onset type 2 diabetes (age, 27+/-5.9 years) was extracted, and the MODY3 gene was amplified. The amplified DNA was hybridized onto a MODY3 chip, which has oligonucleotides of 15-25 bases, representing wild-type and mutant MODY3 sequences in both forward and reverse orientations, immobilized on its surface. RESULTS: Among the normal subjects, there was no mutation of MODY3. Among those with early-onset type 2 diabetes, there was one case of MODY3 mutation. CONCLUSIONS: Our results indicate that MODY3 mutations are not rare in Korean early-onset type 2 diabetes patients in Korea and suggest that MODY3 mutations in patients with early-onset type 2 diabetes need to be further evaluated.
Palabras clave
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Estudios de Casos y Controles
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Prevalencia
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Estudios de Cohortes
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Edad de Inicio
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Pueblo Asiatico
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Diabetes Mellitus Tipo 2
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Factor Nuclear 1-alfa del Hepatocito
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Corea (Geográfico)
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Mutación
Tipo de estudio:
Etiology_studies
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Incidence_studies
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Observational_studies
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Prevalence_studies
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Risk_factors_studies
Límite:
Adolescent
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Adult
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Female
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Humans
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Male
País/Región como asunto:
Asia
Idioma:
En
Revista:
The Korean Journal of Internal Medicine
Año:
2008
Tipo del documento:
Article