Advances of development and application amino acid biosensors / 生物工程学报
Chinese Journal of Biotechnology
; (12): 2485-2501, 2023.
Article
en Zh
| WPRIM
| ID: wpr-981213
Biblioteca responsable:
WPRO
ABSTRACT
Amino acids are the basic building blocks of protein that are very important to the nutrition and health of humans and animals, and widely used in feed, food, medicine and daily chemicals. At present, amino acids are mainly produced from renewable raw materials by microbial fermentation, forming one of the important pillar industries of biomanufacturing in China. Amino acid-producing strains are mostly developed through random mutagenesis- and metabolic engineering-enabled strain breeding combined with strain screening. One of the key limitations to further improvement of production level is the lack of efficient, rapid, and accurate strain screening methods. Therefore, the development of high-throughput screening methods for amino acid strains is very important for the mining of key functional elements and the creation and screening of hyper-producing strains. This paper reviews the design of amino acid biosensors and their applications in the high-throughput evolution and screening of functional elements and hyper-producing strains, and the dynamic regulation of metabolic pathways. The challenges of existing amino acid biosensors and strategies for biosensor optimization are discussed. Finally, the importance of developing biosensors for amino acid derivatives is prospected.
Palabras clave
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Técnicas Biosensibles
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China
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Ensayos Analíticos de Alto Rendimiento
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Ingeniería Metabólica
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Aminoácidos
Límite:
Animals
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Humans
País/Región como asunto:
Asia
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Año:
2023
Tipo del documento:
Article