Preparation and immunogenicity evaluation of mRNA vaccine against porcine epidemic diarrhea / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 2624-2633, 2023.
Artículo
en Chino
| WPRIM
| ID: wpr-981220
ABSTRACT
Porcine epidemic diarrhea (PED) is a highly contagious disease that causes high mortality in suckling piglets. Although several licensed inactivated and live attenuated vaccines were widely used, the infection rate remains high due to unsatisfactory protective efficacy. In this study, mRNA vaccine candidates against PED were prepared, and their immunogenicity was evaluated in mice and pregnant sows. The mRNA PED vaccine based on heterodimer of viral receptor binding region (RBD) showed good immunogenicity. It elicited robust humoral and cellular immune responses in mice, and the neutralizing antibody titer reached 1300 after a single vaccination. Furthermore, it induced neutralizing antibody level similar to that of the inactivated vaccine in pregnant sows. This study developed a new design of PED vaccine based on the mRNA-RBD strategy and demonstrated the potential for clinical application.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Porcinos
/
Enfermedades de los Porcinos
/
Vacunas Atenuadas
/
Vacunas Virales
/
Diarrea
/
Anticuerpos Neutralizantes
/
Anticuerpos Antivirales
Límite:
Animales
/
Embarazo
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2023
Tipo del documento:
Artículo
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