Soybean GmGolS2-2 improves drought resistance of transgenic tobacco / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 2762-2771, 2023.
Artículo
en Chino
| WPRIM
| ID: wpr-981231
ABSTRACT
Galactinol synthase (GolS) genes play important roles in plant response to abiotic stress. In this research, the plant expression vector of soybean GmGolS2-2 gene was constructed and transformed into tobacco to study the drought tolerance of transgenic tobacco. A GmGolS2-2 gene with 975 bp coding sequence was cloned from soybean leaves by reverse transcription-polymerase chain reaction (RT-PCR). GmGolS2-2 was linked to the plant expression vector pRI101 by restriction enzyme sites Nde Ⅰ and EcoR Ⅰ, and transformed into tobacco by leaf disc method. Genomic DNA PCR and real-time PCR showed that three GmGolS2-2 transgenic tobacco plants were obtained. The growth status of GmGolS2-2 transgenic tobacco under drought stress was better than that of wild-type tobacco. After drought stress treatment, the electrolyte leakage and malondialdehyde content of transgenic tobacco were lower than those of wild-type tobacco, but the proline content and soluble sugar content were higher than those of wild-type tobacco. The results of real-time PCR showed that the heterologous expression of GmGolS2-2 increased the expression of stress-related genes NtERD10C and NtAQP1 in transgenic tobacco. The above results indicated that GmGolS2-2 improved drought resistance of transgenic tobacco.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Proteínas de Plantas
/
Glycine max
/
Estrés Fisiológico
/
Nicotiana
/
Plantas Modificadas Genéticamente
/
Regulación de la Expresión Génica de las Plantas
/
Sequías
/
Resistencia a la Sequía
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2023
Tipo del documento:
Artículo
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