Soybean GmGolS2-2 improves drought resistance of transgenic tobacco / 生物工程学报
Chinese Journal of Biotechnology
; (12): 2762-2771, 2023.
Article
en Zh
| WPRIM
| ID: wpr-981231
Biblioteca responsable:
WPRO
ABSTRACT
Galactinol synthase (GolS) genes play important roles in plant response to abiotic stress. In this research, the plant expression vector of soybean GmGolS2-2 gene was constructed and transformed into tobacco to study the drought tolerance of transgenic tobacco. A GmGolS2-2 gene with 975 bp coding sequence was cloned from soybean leaves by reverse transcription-polymerase chain reaction (RT-PCR). GmGolS2-2 was linked to the plant expression vector pRI101 by restriction enzyme sites Nde Ⅰ and EcoR Ⅰ, and transformed into tobacco by leaf disc method. Genomic DNA PCR and real-time PCR showed that three GmGolS2-2 transgenic tobacco plants were obtained. The growth status of GmGolS2-2 transgenic tobacco under drought stress was better than that of wild-type tobacco. After drought stress treatment, the electrolyte leakage and malondialdehyde content of transgenic tobacco were lower than those of wild-type tobacco, but the proline content and soluble sugar content were higher than those of wild-type tobacco. The results of real-time PCR showed that the heterologous expression of GmGolS2-2 increased the expression of stress-related genes NtERD10C and NtAQP1 in transgenic tobacco. The above results indicated that GmGolS2-2 improved drought resistance of transgenic tobacco.
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Asunto principal:
Proteínas de Plantas
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Glycine max
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Estrés Fisiológico
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Nicotiana
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Plantas Modificadas Genéticamente
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Regulación de la Expresión Génica de las Plantas
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Sequías
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Resistencia a la Sequía
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Año:
2023
Tipo del documento:
Article