Resveratrol improves cognitive function in severely burned rats by inhibiting hippocampal NF-κB/JNK pathway / 细胞与分子免疫学杂志
Chinese Journal of Cellular and Molecular Immunology
;
(12): 604-609, 2023.
Artículo
en Chino
| WPRIM
| ID: wpr-981906
ABSTRACT
Objective To investigate the protective effect of resveratrol (RSV) on improving cognitive function in severely burned rats and its possible mechanism. Methods 18 male SD rats aged 18-20 months were randomly divided into 3 groups control group, model group and RSV group, with 6 rats in each group. After successful modeling, the rats in RSV group were gavaged once daily with RSV (20 mg/kg). Meanwhile, the rats in control group and model group were gavaged once daily with an equal volume of sodium chloride solution. After 4 weeks, the cognitive function of all rats was estimated by Step-down Test. The concentration of tumor necrosis factor α (TNF-α) and interleukin 6 (IL-6) protein in serum of rats were detected by ELISA. The expression of IL-6, TNF-α mRNA and protein were estimated by real-time PCR and Western blotting. The apoptosis of hippocampal neurons was tested by terminal deoxynuclectidyl transferase-mediated dUTP-biotin nick end labeling assay (TUNEL). The expression of nuclear transcription factor-κB (NF-κB)/c-Jun N-terminal kinase (JNK) pathway-related proteins in hippocampus were assessed by Western blotting. Results Compared with the rats in model group, rats in RSV group exhibited improved cognitive function. Consistently, the rats in RSV group had a reduced concentration of TNF-α and IL-6 in serum, decreased mRNA and protein expressions of TNF-α and IL-6 in hippocampus, and decreased apoptosis rate and relative expression of p-NF-κB p65/NF-κB p65 and p-JNK/JNK in hippocampal neurons. Conclusion RSV alleviates inflammatory response and hippocampal neuronal apoptosis by inhibiting NF-κB/JNK pathway, thereby improving cognitive function in severely burned rats.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Quemaduras
/
FN-kappa B
/
Interleucina-6
/
Factor de Necrosis Tumoral alfa
/
Ratas Sprague-Dawley
/
Apoptosis
/
Cognición
/
Sistema de Señalización de MAP Quinasas
/
Resveratrol
/
Hipocampo
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Cellular and Molecular Immunology
Año:
2023
Tipo del documento:
Artículo
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