Bilineage embryo-like structure from EPS cells can produce live mice with tetraploid trophectoderm
Protein & Cell
;
(12): 262-278, 2023.
Artículo
en Inglés
| WPRIM
| ID: wpr-982545
ABSTRACT
Self-organized blastoids from extended pluripotent stem (EPS) cells possess enormous potential for investigating postimplantation embryo development and related diseases. However, the limited ability of postimplantation development of EPS-blastoids hinders its further application. In this study, single-cell transcriptomic analysis indicated that the "trophectoderm (TE)-like structure" of EPS-blastoids was primarily composed of primitive endoderm (PrE)-related cells instead of TE-related cells. We further identified PrE-like cells in EPS cell culture that contribute to the blastoid formation with TE-like structure. Inhibition of PrE cell differentiation by inhibiting MEK signaling or knockout of Gata6 in EPS cells markedly suppressed EPS-blastoid formation. Furthermore, we demonstrated that blastocyst-like structures reconstituted by combining the EPS-derived bilineage embryo-like structure (BLES) with either tetraploid embryos or tetraploid TE cells could implant normally and develop into live fetuses. In summary, our study reveals that TE improvement is critical for constructing a functional embryo using stem cells in vitro.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Blastocisto
/
Diferenciación Celular
/
Desarrollo Embrionario
/
Embrión de Mamíferos
/
Tetraploidía
Límite:
Animales
/
Embarazo
Idioma:
Inglés
Revista:
Protein & Cell
Año:
2023
Tipo del documento:
Artículo
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