Experiment study of c-fos expression on myocardial acute ischemia/reperfusion injury in rats / 法医学杂志

ABSTRACT

OBJECTIVE@#To investigate the changes of c-fos mRNA induced by myocardial ischemia/reperfusion(I/R) during acute period.@*METHODS@#The model of I/R was established, and the rats was divided normal control, ischmia and I/R groups. Insitu hybridization (ISH) and computerized image analysis method was used to observe alteration of c-fos mRNA in cardiac myocytes.@*RESULTS@#After 10 min ischemia and 30 min reperfusion, the area of reperfusion showed a part of cardiac myocytes weak staining, and get to peak at I-60 min/R-30 min. The myocardium in normal control and ischemia groups less than 60 min showed negative staining. No changes were found in all groups by HE staining.@*CONCLUSION@#c-fos mRNA detection may become an important method for diagnosis of I/R.