Experiment study of c-fos expression on myocardial acute ischemia/reperfusion injury in rats / 法医学杂志
Journal of Forensic Medicine
; (6): 65-67, 2003.
Article
en Zh
| WPRIM
| ID: wpr-982971
Biblioteca responsable:
WPRO
ABSTRACT
OBJECTIVE@#To investigate the changes of c-fos mRNA induced by myocardial ischemia/reperfusion(I/R) during acute period.@*METHODS@#The model of I/R was established, and the rats was divided normal control, ischmia and I/R groups. Insitu hybridization (ISH) and computerized image analysis method was used to observe alteration of c-fos mRNA in cardiac myocytes.@*RESULTS@#After 10 min ischemia and 30 min reperfusion, the area of reperfusion showed a part of cardiac myocytes weak staining, and get to peak at I-60 min/R-30 min. The myocardium in normal control and ischemia groups less than 60 min showed negative staining. No changes were found in all groups by HE staining.@*CONCLUSION@#c-fos mRNA detection may become an important method for diagnosis of I/R.
Texto completo:
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Índice:
WPRIM
Asunto principal:
ARN Mensajero
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Daño por Reperfusión Miocárdica
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Distribución Aleatoria
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Proteínas Proto-Oncogénicas c-fos
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Ratas Sprague-Dawley
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Hibridación in Situ
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Miocitos Cardíacos
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Medicina Legal
Límite:
Animals
Idioma:
Zh
Revista:
Journal of Forensic Medicine
Año:
2003
Tipo del documento:
Article