Immunohistochemical and morphometrical study on the expression of interleukin-10 (IL-10) in different expressive parts during cutaneous wound healing in mice / 法医学杂志
Journal of Forensic Medicine
; (6): 70-72, 2004.
Article
en Zh
| WPRIM
| ID: wpr-983018
Biblioteca responsable:
WPRO
ABSTRACT
OBJECTIVE@#To explore the expression of interleukin-10 (IL-10) in different expressive parts and its relationship with the wound age during cutaneous wound healing in mice.@*METHODS@#Immunohistochemical and morphometrical analysis methods were performed on vital skin wounds.@*RESULTS@#In normal and injury mouse skin IL-10 immunoreactivity was observed in epidermal cells. The expression of IL-10 in epidermal cells was already enhanced since 1-3h after incision, and it returned to lower lever by 24h, but increased again at 48h after injury. IL-10 immunoreactivive cells under epidermis were mainly infiltrating mononuclear cells. Morphometrically, the ratio of the number of infiltrating cells was evaluated. A subsequent increase in the IL-10 positive cells was observed in the granulation tissue beyond the wound and in dermal at 6h after incision, and the maximum ratio was (51.41 +/- 3.12)% in a 72h-old wound. Then the positive cells gradually decreased (29.38 +/- 2.64)%-(5.56 +/- 4.74)% after incision 96-168h.@*CONCLUSIONS@#The expression characteristics of IL-10 in different expressive parts should be related to the wound age and morphometry of different expressive parts may be employed for determining the wound age.
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Piel
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Coloración y Etiquetado
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Factores de Tiempo
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Cicatrización de Heridas
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Heridas y Lesiones
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Inmunohistoquímica
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Interleucina-10
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Citometría de Flujo
Límite:
Animals
Idioma:
Zh
Revista:
Journal of Forensic Medicine
Año:
2004
Tipo del documento:
Article