Estimation of postmortem intervals by using terminal deoxynucleotidyl transferase

Lan ZHAN; Zi-qin ZHAO; Yi-wen SHAN

Estimation of postmortem intervals by using terminal deoxynucleotidyl transferase / 法医学杂志

Lan ZHAN; Zi-qin ZHAO; Yi-wen SHAN.

Journal of Forensic Medicine ; (6): 113-114, 2005.

Artículo en Chino | WPRIM | ID: wpr-983085

ABSTRACT

ABSTRACT

OBJECTIVE@#To investigate the relationship between DNA degradeation and postmortem interval.@*METHODS@#dUTP was transferred to 3'terminal of DNA by using terminal deoxynucleotidyl transferase (TDT), then the reminders of dUTP after experimental reaction, as indicator of quantity of DNA degradation, were detected.@*RESULTS@#The reminders of dUTP were decreasing along with the postmortem interval.@*CONCLUSION@#Postmortem DNA degradation may be used in postmortem interval judgment.

Asunto(s)

Animales; Masculino; Ratas; Núcleo Celular/metabolismo; Pruebas Enzimáticas Clínicas/métodos; ADN/metabolismo; ADN Nucleotidilexotransferasa/metabolismo; Riñón/metabolismo; Hígado/metabolismo; Cambios Post Mortem; Pirofosfatasas/metabolismo; Ratas Sprague-Dawley; Espectrofotometría Ultravioleta; Bazo/metabolismo; Factores de Tiempo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Cambios Post Mortem / Pirofosfatasas / Espectrofotometría Ultravioleta / Bazo / Factores de Tiempo / ADN / Núcleo Celular / Ratas Sprague-Dawley / ADN Nucleotidilexotransferasa / Pruebas Enzimáticas Clínicas Límite: Animales Idioma: Chino Revista: Journal of Forensic Medicine Año: 2005 Tipo del documento: Artículo

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