Preliminary application on test paper of gold-labelled antibody against fibronectin EIIIA splicing variant / 法医学杂志
Journal of Forensic Medicine
;
(6): 81-83, 2006.
Artículo
en Chino
| WPRIM
| ID: wpr-983142
ABSTRACT
OBJECTIVE@#To develop the test Paper of gold-labelled antibody against fibronectin EIIIR A splicing variant, which can be available in forensic wound interval estimation.@*METHODS@#Two sensitive antibodies were compared with enzyme link immunoabsorband assay (ELISA). After colloid gold labeled, the effects of the two antibodies were tested by methods of Dot immunogold filtration assay and gold immunochromatography assay, respectively. The test paper was finally appraised by applied in experimental skin injury in rats.@*RESULTS@#On the test paper, detected line appeared in three hours wound age group, and then the darkness of positive staining became darker with injury time prolonging, while control normal skin cannot found to be positive staining.@*CONCLUSION@#The gold-labeled test paper can be useful in estimation of wounding interval in forensic science.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Tiras Reactivas
/
Piel
/
Factores de Tiempo
/
Heridas no Penetrantes
/
Ensayo de Inmunoadsorción Enzimática
/
Inmunoensayo
/
Distribución Aleatoria
/
Fibronectinas
/
Ratas Sprague-Dawley
/
Empalme Alternativo
Límite:
Animales
Idioma:
Chino
Revista:
Journal of Forensic Medicine
Año:
2006
Tipo del documento:
Artículo
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