Homologous amelogenin gene test of archaeological samples / 法医学杂志
Journal of Forensic Medicine
;
(6): 213-216, 2006.
Artículo
en Chino
| WPRIM
| ID: wpr-983184
ABSTRACT
OBJECTIVE@#Based on the sequence differences of Amelogenin homologous gene in the X and Y chromosomes, a pair of specific primers was designed to identify the sex of archaeological samples.@*METHODS@#Ancient DNA fragments were extracted from the bones and teeth of sacrificial slaves with an improved method that combines phenol-chloroform extraction, silicon dioxide adsorption with ultrafiltration concentration. The polyacrylamide gel electrophoresis (PAGE) was used to detect PCR products.@*RESULTS@#Seven in sixteen samples from eight graves showed positive results and the targeted segments were visible a male with two bands of 106bp (Amel-X) and 112 bp (Amel-Y), while a female with only one band of 106 bp (Amel-X). Ancient DNA analyzing results from tooth samples are more marked than that from bones.@*CONCLUSION@#The improved extraction method is more effective for ancient DNA extraction, which reduced the PCR inhibitors and lowered experimental costs. The sex determination technology based on Amelogenin homologous gene is an important and feasible method in the molecular archaeological research.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Arqueología
/
Análisis para Determinación del Sexo
/
Diente
/
Huesos
/
ADN
/
Datos de Secuencia Molecular
/
Amplificación de Genes
/
Reacción en Cadena de la Polimerasa
/
Análisis de Secuencia de ADN
/
Cartilla de ADN
Límite:
Femenino
/
Humanos
/
Masculino
Idioma:
Chino
Revista:
Journal of Forensic Medicine
Año:
2006
Tipo del documento:
Artículo
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