Expression of HIF1-alpha on myocardium and lung in rats model of asphyxia death / 法医学杂志
Journal of Forensic Medicine
;
(6): 407-410, 2006.
Artículo
en Chino
| WPRIM
| ID: wpr-983234
ABSTRACT
OBJECTIVE@#To investigate the expression of HIF1-alpha in heart and lung tissue died from asphyxia.@*METHODS@#The rats model of asphyxia death was constructed by hanging, different asphyxia groups and control group sets were made according the postmortem time (0,2,6,24 h), immunohistochemistry and half-quantitative RT-PCR methods were used to investigate expression of HIF1-alpha and mRNA changes on heart and lung tissue.@*RESULTS@#The positive staining of HIF1-alpha could be observed in the myocardium and lung tissue. Significant differences were found between the groups of asphyxia and their corresponding control group. HIF1-alpha expression was found in all the asphyxia groups while it was only expressed in the control groups of 2 h, 6 h and 24 h. Nucleic positive staining could be detected in all the asphyxia groups but none was found in the control groups. RT-PCR showed that the expression of mRNA between 0 h asphyxia group and 0 h control group were equal in both cardic muscle and lung, but elevated expression in groups of 2,6,24h compared to their control groups.@*CONCLUSION@#The nuclear positive staining of HIF1-alpha in heart and lung can be a special character of suffocation death.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Asfixia
/
Factores de Tiempo
/
ARN Mensajero
/
Inmunohistoquímica
/
Distribución Tisular
/
Distribución Aleatoria
/
Ratas Sprague-Dawley
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
/
Hipoxia-Isquemia Encefálica
/
Modelos Animales de Enfermedad
Límite:
Animales
Idioma:
Chino
Revista:
Journal of Forensic Medicine
Año:
2006
Tipo del documento:
Artículo
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