Haplotype and heteroplasmy in the HV II region of mtDNA in human blood detected by PCR-DGGE / 法医学杂志
Journal of Forensic Medicine
;
(6): 30-32, 2007.
Artículo
en Chino
| WPRIM
| ID: wpr-983258
ABSTRACT
OBJECTIVE@#To establish a simple and effective technique for detecting haplotype and heteroplasmy of mtDNA, and investigate their frequencies in Chinese Han population.@*METHODS@#The fragments from 29-290 nt of mtDNA HV II from peripheral leukocytes of 200 unrelated Wuhan Han individuals were analyzed by using PCR-DGGE technique.@*RESULTS@#Seventeen haplotypes were found in the range of 29-290 nt, and the haplotype diversity (HD) was 0.8844. The heterogeneity was observed from 4 individuals, and its frequency was 2%.@*CONCLUSION@#PCR-DGGE is a simple, sensitive and effective technique in analyzing polymorphism and heteroplasmy of mtDNA, and can be used in forensic practice.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Haplotipos
/
ADN Mitocondrial
/
China
/
Reacción en Cadena de la Polimerasa
/
Análisis de Secuencia de ADN
/
Heterogeneidad Genética
/
Pueblo Asiatico
/
Electroforesis en Gel de Poliacrilamida
/
Genética de Población
/
Mutación
Límite:
Humanos
País/Región como asunto:
Asia
Idioma:
Chino
Revista:
Journal of Forensic Medicine
Año:
2007
Tipo del documento:
Artículo
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