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Sequence-length polymorphism research of C-stretch in mitochondrial DNA hyper-variant region / 法医学杂志
Journal of Forensic Medicine ; (6): 123-126, 2007.
Artículo en Chino | WPRIM | ID: wpr-983281
ABSTRACT
OBJECTIVE@#To investigate the polymorphism of C-stretch in mitochondrial DNA hyper-variant region and the values in forensic science.@*METHODS@#The gene frequency were detected in such two loci of nt16180 and nt310 by using PCR-sequencing technique with two pair of primers according to the reference.@*RESULTS@#The length of PCR products were 807 bp and 962 bp respectively. Seven genotypes in each locus were detected. AAAACCCCCTCCCC allele amounted to 87.72% of all nt16180 genotype, while AAAACCCCCCCCCCCCC was firstly reported in Han population. CCCCCCCCTCCCCCC allele amounted to 60.53% of all nt310 genotype. 15 haplotypes were detected among 114 samples. The GD value was 0.6309. The AAAACCCCCTCCCC-CCCCCCCCTCCCCCC haplotype was the most high frequency and amounted to 57.89%.@*CONCLUSION@#The obtained basic genetic data in two loci of nt16180 and nt310 of mtDNA will be useful for forensic science.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Polimorfismo Genético / Haplotipos / ADN Mitocondrial / China / Reacción en Cadena de la Polimerasa / Análisis de Secuencia de ADN / Cartilla de ADN / Alelos / Frecuencia de los Genes / Genética de Población Límite: Humanos País/Región como asunto: Asia Idioma: Chino Revista: Journal of Forensic Medicine Año: 2007 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Polimorfismo Genético / Haplotipos / ADN Mitocondrial / China / Reacción en Cadena de la Polimerasa / Análisis de Secuencia de ADN / Cartilla de ADN / Alelos / Frecuencia de los Genes / Genética de Población Límite: Humanos País/Región como asunto: Asia Idioma: Chino Revista: Journal of Forensic Medicine Año: 2007 Tipo del documento: Artículo