Simultaneous screening for 22 poisonous alkaloids in blood by liquid chromatography-tandem mass spectrometry with multiple-reaction monitoring / 法医学杂志
Journal of Forensic Medicine
; (6): 349-352, 2007.
Article
en Zh
| WPRIM
| ID: wpr-983318
Biblioteca responsable:
WPRO
ABSTRACT
OBJECTIVE@#To establish a liquid chromatography-tandem mass chromatography (LC-MS/MS) method for the simultaneous screening for 22 poisonous alkaloids in blood.@*METHODS@#This method involves a liquid-liquid extraction (LLE) followed by liquid chromatography-tandem mass spectrometry with multi-ple-reaction monitoring (MRM). After blood was extracted with buprenorphine as the internal standard, the target compounds were analyzed with LC-MS/MS-ESI in the positive ionization mode.@*RESULTS@#Identification was based on the compound's retention time and two precursor-to-product ion transitions. The limits of detection ranged from 0.1 ng/mL to 20 ng/mL in blood.@*CONCLUSION@#The method was sufficiently selective and sensitive to detect poisonous alkaloids and can be applied in forensic and clinical toxicology.
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Estricnina
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Aconitina
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Colchicina
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Cromatografía Liquida
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Sensibilidad y Especificidad
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Alcaloides
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Espectrometría de Masas en Tándem
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Medicina Legal
Límite:
Humans
Idioma:
Zh
Revista:
Journal of Forensic Medicine
Año:
2007
Tipo del documento:
Article