Application of immunofluorescence and sandwich ELISA with double-antibodies in detection of human rabies / 法医学杂志
Journal of Forensic Medicine
;
(6): 411-413, 2007.
Artículo
en Chino
| WPRIM
| ID: wpr-983328
ABSTRACT
OBJECTIVE@#To explore the application of immunofluorescence and sandwich ELISA with double-antibodies in detection of human rabies.@*METHODS@#The cerebrum, cerebellum, brainstem, and hippocampus of four patients died of rabies identified by clinical diagnosis were collected and kept in freezer at -70 degrees C or in formaldehyde solution separately. The rat brain tissue infected by CVS strain of rabies virus was used as a positive control and the brain tissue of a patient died of acute pancreatitis was used as a negative control.@*RESULTS@#Rabies virus was detected in the tissues kept in freezer at -70 degrees C and the positive control but was not detected in the tissues kept in formaldehyde solution and the negative control.@*CONCLUSION@#Immunofluorescence and Sandwich ELISA with double-antibodies could be used in detection of human rabies. The samples should be kept in deep frozen temperature condition instead of in formaldehyde solution.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Rabia
/
Virus de la Rabia
/
Juego de Reactivos para Diagnóstico
/
Conservación de Tejido
/
Encéfalo
/
Ensayo de Inmunoadsorción Enzimática
/
Técnica del Anticuerpo Fluorescente
/
Sensibilidad y Especificidad
/
Hipocampo
/
Anticuerpos Antivirales
Límite:
Animales
/
Humanos
Idioma:
Chino
Revista:
Journal of Forensic Medicine
Año:
2007
Tipo del documento:
Artículo
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