Age-dependent mitochondrial DNA 4977bp depletion in human skeletal muscle / 法医学杂志
Journal of Forensic Medicine
; (6): 438-440, 2007.
Article
en Zh
| WPRIM
| ID: wpr-983335
Biblioteca responsable:
WPRO
ABSTRACT
OBJECTIVE@#To explore depletion of human mitochondrial DNA 4977-bp and its relation with aging.@*METHODS@#Total DNA (nuclear and mtDNA) was extracted from 100mg muscle tissue. UV light illumination of ethidium bromide-stained PCR products was used to study the depletion of mtDNA (wild-type or mutant).@*RESULTS@#The proportions of mtDNA depletion in human skeletal muscle could be determined. The frequency of mtDNA 4977-bp depletion in different age groups (0-9, 10-19, 20-29, 30-39, 40-49, 50-59, 60-69, 70-79, 80-89, 90-99) was: 0%, 0%, 0.003%, 0.011%, 0.015%, 0.033%, 0.038%, 0.062%, 0.069%, and 0.091%, respectively.@*CONCLUSION@#Our findings suggest that the frequency of the mtDNA4977 depletion in human skeletal muscle increases with age. It might be useful for human age estimation.
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Envejecimiento
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ADN Mitocondrial
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Reacción en Cadena de la Polimerasa
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Factores de Edad
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Eliminación de Secuencia
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Cartilla de ADN
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Músculo Esquelético
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Mitocondrias Musculares
Límite:
Adolescent
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Adult
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Aged
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Aged80
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Child
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Child, preschool
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Female
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Humans
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Infant
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Male
Idioma:
Zh
Revista:
Journal of Forensic Medicine
Año:
2007
Tipo del documento:
Article