DNA polymorphism detection of Papaver somniferum L using fluorescent amplified fragment length polymorphism / 法医学杂志
Journal of Forensic Medicine
;
(6): 262-267, 2008.
Artículo
en Chino
| WPRIM
| ID: wpr-983391
ABSTRACT
OBJECTIVE@#To detect DNA polymorphism of Papaver somniferum L using fluorescent Amplified Fragment Length Polymorphism.@*METHODS@#Genomic DNA was isolated using the AxyPrep DNA Kit, double-digested by two restrictional endonucleases (Eco RI and Mse I) and ligated to oligonucleotide adapters. After Pre-amplification and selective amplification, the DNA fragments were separated by capillary electrophoresis using the CEQ8000 DNA Fragment Analyzer.@*RESULTS@#More than 20 fragments of highly polymorphic products were obtained by 8 pairs of primer from 64 selective amplifying primer pairs.@*CONCLUSION@#The fluorescent AFLP technique can be used to detect the DNA polymorphism of Papaver somniferum.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Papaver
/
Polimorfismo Genético
/
ADN de Plantas
/
Genética Forense
/
Análisis del Polimorfismo de Longitud de Fragmentos Amplificados
/
Colorantes Fluorescentes
Idioma:
Chino
Revista:
Journal of Forensic Medicine
Año:
2008
Tipo del documento:
Artículo
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